| Audio Recording Technology Institute | Private, Non-profit, Less than 2 years | Non-degree granting | Orlando, 32811 |
| Career Training Institute | Private, Non-profit, Less than 2 years | Non-degree granting | Orlando, 32804 |
| DeVry University-Florida | Private, Non-profit, 4 years or more | Masters | Orlando, 32839 |
| Florida College of Integrative Medicine | Private, Non-profit, 4 years or more | Masters | Orlando, 32809 |
| Florida Hospital College of Health Sciences | Private, For-profit, 4 years or more | Bachelors | Orlando, 32803 |
| Florida Hospital School of Medical Technology | Private, Non-profit, 4 years or more | Bachelors | Orlando, 32803 |
| Florida Metropolitan University-North Orlando | Private, Non-profit, 4 years or more | Masters | Orlando, 32810 |
| Florida Metropolitan University-South Orlando | Private, Non-profit, 4 years or more | Masters | Orlando, 32819 |
| Florida Technical College | Private, Non-profit, 2 to 4 years | Associates | Orlando, 32826 |
| High-Tech Institute-Orlando | Private, Non-profit, 2 to 4 years | Associates | Orlando, 32803 |
| International Academy of Design and Technology | Private, Non-profit, 4 years or more | Bachelors | Orlando, 32809 |
| Mid Florida Tech | Public, 2 to 4 years | Non-degree granting | Orlando, 32809 |
| Orlando Culinary Academy | Private, Non-profit, 2 to 4 years | Associates | Orlando, 32819 |
| Orlando Tech | Public, Less than 2 years | Non-degree granting | Orlando, 32801 |
| Polytechnic Institute of America | Private, Non-profit, Less than 2 years | Non-degree granting | Orlando, 32810 |
| Southern Technical Institute | Private, Non-profit, Less than 2 years | Non-degree granting | Orlando, 32807 |
| Stenotype Institute of Jacksonville Inc | Private, Non-profit, 2 to 4 years | Non-degree granting | Orlando, 32809 |
| Universal Technical Institute-Automotive, Motorcycle & Marine Mechanics | Private, Non-profit, 2 to 4 years | Non-degree granting | Orlando, 32837 |
| University of Central Florida | Public, 4 years or more | Doctoral | Orlando, 32816 |
| Valencia Community College | Public, 2 to 4 years | Associates | Orlando, 32801 |